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Multifunctional Bi-layer collagen nanofiber-collagen/PLLA/Zataria multifloraessential oil nanofiber for wound healing: Antibacterial, antifungal and antioxidant properties
- Source :
- Journal of Drug Delivery Science and Technology; February 2024, Vol. 92 Issue: 1
- Publication Year :
- 2024
-
Abstract
- Multi-layer electrospun structures have attracted interests as multifunctional wound dressings. In the present study, a double-layered nanofibrous construct was prepared out of pure type I collagen nanofibers (as the inner layer) and collagen/PLLA/Zataria Multifloraessential oil (as the outer layer). Extracted essential oil was analyzed by GC–Mass Spectrometry. Bi-layers were characterized by SEM, ATR-FTIR and XRD. Water contact angle and swelling studies demonstrated hydrophilicity and a high water absorption capability (1504% of the initial weight) of the nanofibers, respectively. Also, the essential oil showed a strong antioxidant activity (IC50: 7.4 ppm). Cumulative release profile of the essential oil from nanofibers illustrated a burst release in about 120 min and then, a sustained release up to 72 h. Broth micro-dilution, disk diffusion and AATCC 100 assays were performed and the bi-layer dressings exhibited strong antibacterial activity (against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosaand Escherichia coli) and antifungal activity against 4 Candidaspecies. Viability of the fibroblast cells on the obtained structures was evaluated by alamar blue and live/dead assays that confirmed appropriate cyto-compatibility of the nanofibers. Therefore, biocompatible double-layered constructs with strong antimicrobial, antioxidant and water absorption capabilities would be promising as potential wound dressings.
Details
- Language :
- English
- ISSN :
- 17732247
- Volume :
- 92
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Journal of Drug Delivery Science and Technology
- Publication Type :
- Periodical
- Accession number :
- ejs65031356
- Full Text :
- https://doi.org/10.1016/j.jddst.2023.105285