Identification of novel canonical and cryptic HCMV-specific T-cell epitopes for HLA-A∗03 and HLA-B∗15 via peptide-PRISM

•HCMV-derived peptides for HLA-A∗03:01 and HLA-B∗15:01 haplotypes were identified via Ribo-seq, mass spectrometry, and machine learning.•Six novel immunogenic peptides were identified, establishing a framework for efficient detection of peptides from Ribo-seq data sets.