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Fecal virus transplantation has more moderate effect than fecal microbiota transplantation on changing gut microbial structure in broiler chickens
- Source :
- Poultry Science; February 2024, Vol. 103 Issue: 2
- Publication Year :
- 2024
-
Abstract
- Growing evidence of fecal microbiota transplantation (FMT) and fecal virus transplantation (FVT) provides a possibility to regulate animal health, whereas little is known about the impact of the 2 methods. This study aimed to investigate the effects of gut microbes on jejunal function in healthy broiler chickens, with the objective of establishing a theoretical basis for the application of FMT and FVT. Cecal feces from 28-day-old AA broilers were collected to prepare gavage juice for FMT and FVT. FMT for Group FM, FVT for group FV and PBS gavage for group CON, continuously treated for 6 days start at 5-day-old chicks. Samples were collected at d 11 and d 21. The results showed that the treatment d 2 and the overall fecal score in treatment groups were significantly lower than CON group (P< 0.05). The jejunum morphology showed that FMT increased crypt depth, decreased villus height, V/C (P< 0.05) and FVT increased villus height (P< 0.05) at d 11. At d 21, villus height and crypt depth significantly higher (P< 0.05) in group FM and group FV. The expression of Claudin1, Occludin, ZO2, and Muc2in the FV group was significantly increased (P< 0.05) at 11-day-old. FMT increased the secretion of sIgA at 11-day-old, and this influence lasted up to 21-day-old (P< 0.05). At 11-day-old, the expression of b0+ATof basic amino acid transport carrier and chymotrypsin activity (P< 0.05) had a significant correlation. At 21 d of age, FVT significantly increased the expression of PepT1and SGLT1(P< 0.05). At 11-day-old, FM group showed significantly higher faith pd index (P= 0.004) and Shannon index (P= 0.037), and separated from FV and CON according to PCoA. Among differentiating bacteria, Bacteroidessignificantly enriched (P< 0.05) in group FM, which positively correlated with the expression of ZO2, Muc2, Occludin,and Claudin1; R_Ruminococcus, L_Ruminococcus, Butyricicoccusssignificantly enriched (P< 0.05) in group CON, which significantly higher than processing groups, R_Ruminococcusand L_Ruminococcusnegatively correlated with the expression of Occludin(P< 0.05), and R_Ruminococcus, Butyricicoccusnegatively correlated with the expression of Claudin1(P< 0.05). At 21-day-old, PCoA based on Bray-Curtis shows that microbes taxa of 3 groups are isolated with each other and treatment groups were significant different with CON group based on Unweighted UniFrac and weighted UniFrac. The expression of PepT1was significantly negatively (P< 0.05) correlated with Ruminococcus, and the expression of sIgA was significantly negatively (P< 0.05) correlated with Parabacteroides. In conclusion, FMT regulated intestinal flora rapidly, while it had little effect on intestinal function and a higher potential damaging risk on jejunal. FVT regulated intestinal flora structure softer, improved tight junction expression, but the mechanism of action needs further exploration.
Details
- Language :
- English
- ISSN :
- 00325791 and 15253171
- Volume :
- 103
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Poultry Science
- Publication Type :
- Periodical
- Accession number :
- ejs64719334
- Full Text :
- https://doi.org/10.1016/j.psj.2023.103282