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Nitric oxide-releasing albumin nanoclusters facilitate healing of methicillin-resistant Staphylococcus aureus-infected cutaneous wounds
- Source :
- Journal of Pharmaceutical Investigation; 20230101, Issue: Preprints p1-10, 10p
- Publication Year :
- 2023
-
Abstract
- Purpose: Although nitric oxide (NO)-releasing nanoparticles have garnered significant attention owing to their potent antibacterial effects against methicillin-resistant Staphylococcus aureus(MRSA) and direct promotion of wound healing, the majority of NO-releasing nanoparticles are composed of synthetic polymers or inorganic materials, which may lead to unfavorable effects in the body. To overcome this limitation, we developed NO-releasing albumin nanoclusters (NO/ANCs) using bovine serum albumin, a fully biocompatible endogenous material. Methods: NO/ANCs were fabricated by incorporating diethylenetriamine diazeniumdiolate (DT/NO) into albumin nanoclusters via electrostatic interactions, followed by glutaraldehyde crosslinking between the albumin molecules. The antibacterial effects of the NO/ANCs were investigated using the LIVE/DEAD® BacLightâ„¢ bacterial viability kit. The enhanced wound healing and in vivo antibacterial effects were evaluated in a mouse model of MRSA-challenged full-thickness wounds. Results: NO/ANCs were successfully fabricated and could release NO for more than 17 h. NO/ANCs demonstrated enhanced in vitro antibacterial effects and exhibited improved in vivo antibacterial and wound-healing effects in a mouse model of MRSA-challenged full-thickness wounds. Conclusion: NO/ANCs could be a promising formulation for the treatment of MRSA-infected cutaneous wounds.
Details
- Language :
- English
- ISSN :
- 20935552 and 20936214
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Journal of Pharmaceutical Investigation
- Publication Type :
- Periodical
- Accession number :
- ejs64550666
- Full Text :
- https://doi.org/10.1007/s40005-023-00641-y