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Establishment of Biotesting System to Study Features of Innovative Multifunctional Biotextile

Authors :
Grauda, Dace
Butkauskas, Dalius
Vyšniauskienė, Regina
Rančelienė, Vida
KrasņevskA, Nikole
Miķelsone, Andra
Žagata, Kārlis
Gobiņš, Valters
Rashal, Isaak
Rašals, Dāvis
Ignatavičienė, Ieva
Petrosius, Rimantas
Vilcāne, Inese
Ļašenko, Inga
Source :
Proceedings of the Latvia University of Agriculture; August 2023, Vol. 77 Issue: 3-4 p186-192, 7p
Publication Year :
2023

Abstract

An established biotesting system designed to discover specific features of innovative multifunctional biotextile, encompassing integrated silica dioxide and succinite (Baltic amber) particles, is founded on reproducible, reliable, and relatively fast methods. The main idea starting this study was to create a system of test methods devoted to identification of specific features of biotextile materials designed to preserve living organisms from adverse environmental factors like enhanced electromagnetic radiation of different frequencies, without use of vivarium animals. Cultures of the freshwater macrophyte duckweed (Lemna minor) line Sta2and fruit flies (Drosophila melanogaster) were chosen as model systems suitable for the study of the influence of electromagnetic field (EMF) radiation. The experiments showed changes of phenotypic features and growth parameters of test objects, as well as induction of point mutations of DNA (for example, insertions or deletions in chloroplast DNA and nucleotide substitutions in nuclear genes). The responses of test organisms induced by EMF were studied using microscopy, flow cytometry, and DNA sequencing methods. On the cell level, a new fast flow cytometry method for biotextile testing was developed: immature gametic (pollen) cell cultures were used as a highly sensitive model system (plant gametic cell response is comparable to human neutrophil response) to study the influence of EMF radiation. The method was based on the measurement of differences of fluorescence intensity between group of cells experimentally affected by EMF radiation and non-affected cells.

Details

Language :
English
ISSN :
14074427
Volume :
77
Issue :
3-4
Database :
Supplemental Index
Journal :
Proceedings of the Latvia University of Agriculture
Publication Type :
Periodical
Accession number :
ejs64112696
Full Text :
https://doi.org/10.2478/prolas-2023-0026