Efficient clearance of periodontitis pathogens by S. gordoniimembrane-coated H2O2self-supplied nanocomposites in a “Jenga” styleElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3bm00641g

As a key pathogen of periodontitis, P. gingivalisrequires support of the initial colonizing bacterium (S. gordoniipreferably) to form symbiotic biofilms on gingival tissues with enhanced antibiotic resistance. Here, we report a new strategy to treat periodontitis biofilms with S. gordoniimembrane-coated H2O2self-supplied nanocomposites (ZnO2/Fe3O4@MV NPs) in a “Jenga” style. Integration of our special MV coatings enables selectively enhanced internalization of the cargos in S. gordonii, thus inducing severe damage to the foundational bacterial layer and collapse/clearance of symbiotic biofilms consequently. This strategy allows us to clear the symbiotic biofilms of S. gordoniiand P. gingivaliswith active hydroxyl radicals (OH) derived from ZnO2–Fe3O4@MV NPs in a H2O2self-supplied, nanocatalyst-assisted manner. This “Jenga-style” treatment provides a cutting-edge proof of concept for the removal of otherwise robust symbiotic biofilms of periodontitis where the critical pathogens are difficult to target and have antibiotic resistance.