Emergence of NDM-1 and KPC-3 carbapenemases in Kluyvera cryocrescens: Investigating genetic heterogeneity and acquisition routes of blaNDM-1in Enterobacteralesspecies in Portugal

•Emergence of NDM-1 and KPC-3 in the infrequent Kluyvera cryocrescensin Portugal.•Global platforms carrying blaNDM-1(Tn3000) and blaKPC-3(Tn4401d, IncN-ST15).•Heterogeneity of blaNDM-1genetic environments in variable plasmid types.•Multiple acquisition events for blaNDM-1in different Enterobacterales species.•Tracking genetic platforms is crucial to prevent carbapenemases diffusion.