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Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine
- Source :
- Cell Reports; 20230101, Issue: Preprints
- Publication Year :
- 2023
-
Abstract
- Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike+(S-2P+) and S-2P−B cells reveal clonal expansion and accumulating mutations among S-2P+cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3+MBCs. One branch leads to CD11c+atypical MBCs while the other develops from CD71+activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P+clones, several are populated with plasmablasts at early timepoints as well as CD71+activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs.
Details
- Language :
- English
- ISSN :
- 22111247
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Cell Reports
- Publication Type :
- Periodical
- Accession number :
- ejs63421621
- Full Text :
- https://doi.org/10.1016/j.celrep.2023.112780