Bacterial cell-free metabolites-based zinc oxide nanoparticles for combating skin-causing bacterial infections

Recently, the development of skin barrier depend on wound healing, which is one of the most complicated biological processes. As an alternative to conventional antibiotics, nanoparticles (NPs) have become more utilized generally to attack bacteria. Due to their distinct characteristics, potential microbicidal action, and ability to speed up the wound healing process, zinc oxide nanoparticles (ZnO-NPs) have attracted much attention. Biological techniques can solve the restrictions of both physical and chemical approaches for nanoparticles synthesis. Because it does not require expensive chemicals, high temperatures, or a lot of time, biological synthesis is relatively easy, inexpensive, and environmentally benign. The secondary metabolic extract from Escherichia coliwas used in this study to biologically synthesize three distinct quantities of ZnO-NPs, which were then assessed for their effectiveness in wound healing and bacterial infection prevention. The biofabricated ZnO-NPs were fully characterized in terms of particle shape, morphology, and stability against aggregation. Depending on the concentration of the utilized zinc salt, three different samples were fabricated biologically, nominated as ZnO-NPs-1, ZnO-NPs-2, and ZnO-NPs-3. The findings of Uv-vis absorption peaks were obtained at 352 nm, demonstrating the preparation of ZnO-NPs. The results demonstrated the formation of ZnO-NPs with an average particle size of 79.19, 79.83 and 91.57 nm for the three prepared samples (ZnO-NPs-1, ZnO-NPs-2, and ZnO-NPs-3), respectively. Additionally, these samples of ZnO-NPs exhibited zeta potential values around −34.3, −33.7, and −33.4 mV, respectively. Energy dispersive X-ray confirmed the successful formation of ZnO-NPs. It was also observed from the obtained results that, ZnO-NP-3 showed superior antimicrobial potential against selected skin infectious microbes. The effective killing dosage of ZnO-NPs-3 was recorded to be 40 mg/L which can eliminate microbial growth. The dysregulation of skin flora significantly influences the etiology of inflammatory skin disorders.