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Endolysin Inhibits Skin Colonization by Patient-Derived Staphylococcus Aureusand Malignant T-Cell Activation in Cutaneous T-Cell Lymphoma
- Source :
- Journal of Investigative Dermatology; September 2023, Vol. 143 Issue: 9 p1757-1768.e3
- Publication Year :
- 2023
-
Abstract
- Staphylococcus aureusis suspected to fuel disease activity in cutaneous T-cell lymphomas. In this study, we investigate the effect of a recombinant, antibacterial protein, endolysin (XZ.700), on S. aureusskin colonization and malignant T-cell activation. We show that endolysin strongly inhibits the proliferation of S. aureusisolated from cutaneous T-cell lymphoma skin and significantly decreases S. aureusbacterial cell counts in a dose-dependent manner. Likewise, ex vivo colonization of both healthy and lesional skin by S. aureusis profoundly inhibited by endolysin. Moreover, endolysin inhibits the patient-derived S. aureusinduction of IFNγand the IFNγ-inducible chemokine CXCL10in healthy skin. Whereas patient-derived S. aureusstimulates activation and proliferation of malignant T cells in vitro through an indirect mechanism involving nonmalignant T cells, endolysin strongly inhibits the effects of S. aureuson activation (reduced CD25 and signal transducer and activator of transcription 5 phosphorylation) and proliferation (reduced Ki-67) of malignant T cells and cell lines in the presence of nonmalignant T cells. Taken together, we provide evidence that endolysin XZ.700 inhibits skin colonization, chemokine expression, and proliferation of pathogenic S. aureusand blocks their potential tumor-promoting effects on malignant T cells.
Details
- Language :
- English
- ISSN :
- 0022202X and 15231747
- Volume :
- 143
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Journal of Investigative Dermatology
- Publication Type :
- Periodical
- Accession number :
- ejs62435902
- Full Text :
- https://doi.org/10.1016/j.jid.2023.01.039