A Technique for the Cultivation of Insect Tissues

In vitro tissue culture is shown to be a possible mode of experimentation with the tissues of the Blow Fly larva. Methods are described-whereby the tissues, and the body fluids requisite as culture media may be obtained free from bacteria. The imperfections of the technique are noted and the conclusion reached that a successful technique must depend on the rearing of bacteria-free larvae, for which a method is briefly outlined. It is shown that progress in this part of the work must await further physiological knowledge, particularly in respect to the nature of the body fluids.