Impaired immune response and barrier function in GSPD-1-deficient C. elegansinfected with Klebsiella pneumoniae

•Lack of GSPD-1 increased sensitivity to the infection of K. pneumoniaein C. elegans.•The innate immune response was the most important pathway affected by GSPD-1 revealed by RNAseq.•GSPD-1 maintained tight junction and intestinal barrier integrity.•GSPD-1 enhanced innate immunity though regulating the antimicrobial effector, lysozymes.•G6PD-deficient human cell culture recapitulated the defective immune response.