Detection of Mycobacterium tuberculosisand Mycobacterium aviumComplexes by Real-Time PCR in Bovine Milk from Brazilian Dairy Farms

Foodborne diseases are a public health problem worldwide. The consumption of contaminated raw milk has been recognized as a major cause of transmission of bovine tuberculosis to humans. Other mycobacteria that may be present in raw milk and may cause diseases are those belonging to the Mycobacterium aviumcomplex. In this study, molecular biology tools were applied to investigate raw milk contamination with Mycobacteriumspp. in family dairy farms from Rio Grande do Sul, southern Brazil. Furthermore, different variables related to the source of the milk, herd characteristics, and management were evaluated for their effect on milk contamination. Five hundred and two samples were analyzed, of which 354 were from the Northwest region (102 farms with samples from 93 bulk tanks and 261 animals) and 148 from the South region of the state (22 farms with samples from 23 bulk tanks and 125 animals). Among them, 10 (1.99%) and 7 (1.39%) were positive for Mycobacterium tuberculosis(9 confirmed as Mycobacterium bovis) and M. aviumcomplexes, respectively. There was no difference in the frequencies of positive samples between the regions or the sample sources. Of the positive samples, 4 were collected from a bulk tank (1 positive for M. aviumand 3 for M. tuberculosis).Moreover, 1 sample was positive concomitantly for M. tuberculosisand M. aviumcomplexes. On risk analysis, no variable was associated with raw milk contamination by M. tuberculosiscomplex species. However, washing the udders of all animals and drying them with paper towels were weakly classified as risk factors for M. aviumcontamination. Positive samples were obtained from both animals and bulk tanks, which emphasizes the importance of tuberculosis control programs and provides evidence that milk monitoring can be used as a control practice. Moreover, the findings of this study reinforce the need for awareness of the problems of raw milk consumption among the general population.