Back to Search Start Over

(Invited) Finite Element Model Simulations to Assist the Design of Microdevices Dedicated to the Localized Electroporation of Mouse Embryos

Authors :
Zhao, Xuan
Mazari, Elsa
Suárez-Boomgaard, Diana
Migeotte, Isabelle
Perea-Gomez, Aitana
Gosse, Charlie
Source :
ECS Transactions; August 2014, Vol. 64 Issue: 16
Publication Year :
2014

Abstract

We have recently developed a microsystem to electroporate a few cells at the surface of early post-implantation mouse embryos. We could achieve the efficient, reproducible, and safe transfection of various genetic markers, which allowed single cell fate studies during morphogenesis. However, our single-use polymeric device necessitated to be fabricated in a clean room the day before each experiment. Thus, we here introduce an all-glass chip that any biologist can easily recycle in its laboratory. Most importantly, during the technological evolution process we could validate a comprehensive design strategy based on finite element model simulations. Indeed, both the embryo and the microsystem were represented as very simple electric objects and stationary computations enabled to properly predict the voltage pulse amplitude that would yield optimal device performances.

Details

Language :
English
ISSN :
19385862 and 19386737
Volume :
64
Issue :
16
Database :
Supplemental Index
Journal :
ECS Transactions
Publication Type :
Periodical
Accession number :
ejs61757101
Full Text :
https://doi.org/10.1149/06416.0007ecst