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Protective Effect of Conditioned Media of Human Fetal Dermal Mesenchymal Stem Cells Can Inhibit Burn-induced Microvascular Hyperpermeability

Authors :
Pan, Yi
Wang, Xiao
Wang, Xinglei
Shan, Fei
Wang, Maoying
Zhang, Jixun
Zhang, Jingjuan
Jia, Shanshan
Jiao, Ya
Qi, Yongjun
Gong, Hongmin
Jiang, Duyin
Source :
Journal of Burn Care & Research; May 2022, Vol. 43 Issue: 3 p735-741, 7p
Publication Year :
2022

Abstract

Burns often cause loss of skin barrier protection, fluid exudation, and local tissue edema, which hinder functional recovery. Effectively improving the quality of deep burn wound healing, shortening the wound healing time, and reducing tissue fluid leakage are urgent problems in the medical field. Human mesenchymal stem cells (MSCs) can effectively stabilize vascular endothelial injury. Fetal dermal MSCs (FDMSCs) are a newly discovered source of MSCs derived from the skin of accidentally aborted fetuses. However, the effect of FDMSCs on vascular permeability remains poorly understood. In this study, conditioned media from FDMSCs (F-CM) extracted from fetal skin tissue was prepared. The effect of F-CM on vascular permeability was evaluated using the internal circulation method FITC-dextran in vivo, and several in vitro assays, including cell viability assay, transwell permeability test, immunofluorescence, and western blotting. Altogether, our results demonstrate that F-CM could inhibit burn-induced microvascular hyperpermeability by increasing the protein expression levels of occludin and VE-cadherin, while restoring the expression of endothelial F-actin, and providing the foundation of a novel therapy for the treatment of burns with F-CM.

Details

Language :
English
ISSN :
1559047X and 15590488
Volume :
43
Issue :
3
Database :
Supplemental Index
Journal :
Journal of Burn Care & Research
Publication Type :
Periodical
Accession number :
ejs60818614
Full Text :
https://doi.org/10.1093/jbcr/irab194