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Expression and distribution pattern of aggrecanases and miR-140sin the thickened synovia of shoulder joints in rotator cuff tears: A retrospective observational study
- Source :
- Medicine; August 2022, Vol. 101 Issue: 32 pe29583-e29583, 1p
- Publication Year :
- 2022
-
Abstract
- The rotator cuff (RC) is frequently torn at the enthesis composed of fibrocartilage. We aimed to histopathologically evaluate lining layers and assess the distribution of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)4, ADAMTS5, and microRNA (miR)-140sin the synovia of patients with RC tears. We recruited 51 patients who underwent arthroscopic surgical treatment for full-thickness rotator cuff tears, including 26 patients with < 3 cm tear size (group N) and 25 patients with ≥ 3 cm tear size (group W). Biopsied synovia were analyzed using histological and immunohistological techniques for the presence ADAMTS4 and ADAMTS5. The layers of the synovial lining were morphologically classified into 3 grades according to the synovitis score and staining levels of ADAMTSs. The glenohumeral synovia from 8 patients with recurrent shoulder dislocation (group C) were used as controls. Furthermore, in situ hybridization was performed to evaluate the presence of miR-140sin patients with massive tears and recurrent shoulder dislocation. The staining levels were evaluated and analyzed based on comparison between patient groups and correlation between ADAMTS5 and miR-140s. Histological analysis revealed significant differences between groups W and C. ADAMTS5 and ADAMTS4 were strongly expressed in the synovial lining of patients in group W, and this expression was significantly higher than that in groups C and N. In addition, expression of ADAMTS5 was inversely correlated with that of miR-140-3p. This study showed that synovia from group W had a significantly higher rate of severely thickened areas with strong expression of both aggrecanases. Furthermore, the area with weak expression of miR-140-3pshowed strong ADAMTS5 expression.
Details
- Language :
- English
- ISSN :
- 00257974 and 15365964
- Volume :
- 101
- Issue :
- 32
- Database :
- Supplemental Index
- Journal :
- Medicine
- Publication Type :
- Periodical
- Accession number :
- ejs60602738
- Full Text :
- https://doi.org/10.1097/MD.0000000000029583