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Targeting of transgene expression to monocyte/macrophages by the gp91-phox promoter and consequent histiocytic malignancies.

Authors :
Skalnik, D G
Dorfman, D M
Perkins, A S
Jenkins, N A
Copeland, N G
Orkin, S H
Source :
Proceedings of the National Academy of Sciences of the United States of America; October 1991, Vol. 88 Issue: 19 p8505-8509, 5p
Publication Year :
1991

Abstract

A component of a heterodimeric cytochrome b, designated gp91-phox, is required for the microbicidal activity of phagocytic cells and is expressed exclusively in differentiated myelomonocytic cells (granulocytes; monocyte/macrophages). In an attempt to identify cis-elements responsible for this restricted pattern of expression, we produced transgenic mice carrying reporter genes linked to the human gp91-phox promoter. Immunohistochemical and RNA analyses indicate that 450 base pairs of the proximal gp91-phox promoter is sufficient to target reporter expression to a subset of monocyte/macrophages. Mice expressing simian virus 40 large tumor antigen under control of the gp91-phox promoter develop monocyte/macrophage-derived malignancies with complete penetrance at 6-12 mo of age and provide an animal model of true histiocytic lymphoma. As these transgenes are inactive in most phagocytic cells that express the endogenous gp91-phox-encoding gene, we infer that additional genomic regulatory elements are necessary for appropriate targeting to the full complement of phagocytes in vivo.

Details

Language :
English
ISSN :
00278424 and 10916490
Volume :
88
Issue :
19
Database :
Supplemental Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Periodical
Accession number :
ejs60479722
Full Text :
https://doi.org/10.1073/pnas.88.19.8505