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Conversion of B DNA between solution and fiber conformations.

Authors :
Mandelkern, M
Dattagupta, N
Crothers, D M
Source :
Proceedings of the National Academy of Sciences of the United States of America; July 1981, Vol. 78 Issue: 7 p4294-4298, 5p
Publication Year :
1981

Abstract

At low salt and high DNA concentration, rodlike DNA molecules form specific aggregates containing about seven parallel DNA molecules packed in a bundle. Bundle formation can be reversed by addition of small amounts of EDTA, and formation of the specific aggregates can be induced at higher salt concentration by addition of trivalent ions. In contrast to monomeric DNA, the aggregated species has the optical anisotropy expected for the B form as observed in fibers.

Details

Language :
English
ISSN :
00278424 and 10916490
Volume :
78
Issue :
7
Database :
Supplemental Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Periodical
Accession number :
ejs60436668
Full Text :
https://doi.org/10.1073/pnas.78.7.4294