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Drosophila homolog of the murine Int-1 protooncogene.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; May 1988, Vol. 85 Issue: 9 p3034-3038, 5p
- Publication Year :
- 1988
-
Abstract
- We have isolated phage clones from Drosophila melanogaster genomic and cDNA libraries containing a sequence homologous to the murine Int-1 protooncogene. The Drosophila gene is represented by a single locus at position 28A1-2 on chromosome 2. The gene is expressed as a 2.9-kilobase-long polyadenylylated mRNA in embryo, larval, and pupal stages. It is hardly detectable in adult flies. The longest open reading frame of the cDNA clone corresponds to a protein 469 amino acids long. Alignment of the predicted amino acid sequences shows that the Drosophila protein is 86 amino acids longer than its murine counterpart. In spite of the difference in length, the two proteins are highly conserved with an overall sequence homology of 54%. Both Drosophila and murine Int-1 proteins begin with a hydrophobic leader sequence and contain cysteine residues and sites for glycosylation (four in the murine protein and one in the Drosophila protein) in conserved positions, suggesting that they play important functional roles.
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Volume :
- 85
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Periodical
- Accession number :
- ejs60436031
- Full Text :
- https://doi.org/10.1073/pnas.85.9.3034