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15N NMR study of a mixture of uniformly labeled tRNAs.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; August 1982, Vol. 79 Issue: 16 p4834-4837, 4p
- Publication Year :
- 1982
-
Abstract
- 15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Volume :
- 79
- Issue :
- 16
- Database :
- Supplemental Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Periodical
- Accession number :
- ejs60432606
- Full Text :
- https://doi.org/10.1073/pnas.79.16.4834