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Fluorometric Assay of DNA by Its Fluorescent Reaction with Chloroacetic Acid
- Source :
- Analytical Sciences; April 1997, Vol. 13 Issue: 2 p251-254, 4p
- Publication Year :
- 1997
-
Abstract
- A new fluorescent reaction of DNA with chloroacetic acid in the presence of triethylamine is proposed, and the reaction conditions have been optimized. Based on this fluorescent reaction, a sensitive fluorometric method is described for the determination of DNA and the selective determination of cytosine and dCMP. The detection limits for DNA, cytosine and dCMP were 6.0, 0.4 and 1.4 ng/ml, respectively.
Details
- Language :
- English
- ISSN :
- 09106340 and 13482246
- Volume :
- 13
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Analytical Sciences
- Publication Type :
- Periodical
- Accession number :
- ejs60299958
- Full Text :
- https://doi.org/10.2116/analsci.13.251