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Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode
- Source :
- Analytical Sciences; January 2013, Vol. 29 Issue: 1 p79-83, 5p
- Publication Year :
- 2013
-
Abstract
- We fabricated a thermostable quinoprotein aldose sugar dehydrogenase (tPQQ-ASD derived from a hyperthermophilic archaeon Pyrobaculum aerophilum)immobilized electrode. The electrode was prepared by immobilizing agarose gel mixed with the enzyme and carbon nanofiber (CNF) on a carbon paste (CP) electrode containing p-benzoquinone (BQ) as an electron mediator. The electrocatalytic response was clearly observed by the addition of D-glucose at the electrode. The electrode properties such as pH, temperature dependency and substrate selectivity basically followed the enzyme properties. The current response against D-glucose increased with measurement temperatures up to 70°C, and response perturbation caused by dissolved oxygen level was not observed at the electrode. As for the results of long-term stability evaluation, the current response was stable for 30 days when the electrode was stored in HEPES buffer solution (pH 7.0) at 4°C.
Details
- Language :
- English
- ISSN :
- 09106340 and 13482246
- Volume :
- 29
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Analytical Sciences
- Publication Type :
- Periodical
- Accession number :
- ejs60220839
- Full Text :
- https://doi.org/10.2116/analsci.29.79