Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application

A newly designed oxime based probe, 2,4-dihydroxyacetophenone-oxime (DHAO), was found to recognize H2AsO4–selectively with ~3 fold “turn-on” fluorescence enhancement and LOD of 29 μM, K= (2.10 ± 0.54)× 104M–1in purely aqueous medium. The structures of the DHAO and its adduct with H2AsO4–were optimized by DFT calculations. Intracellular imaging of As(V) in HepG2 cells demonstrate the possibilities of in vitro/in vivoapplications for selective monitoring of such species.