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Novel metabolic interactions and environmental conditions mediate the boreal peatmoss-cyanobacteria mutualism

Authors :
Carrell, Alyssa A.
Veličković, Dušan
Lawrence, Travis J.
Bowen, Benjamin P.
Louie, Katherine B.
Carper, Dana L.
Chu, Rosalie K.
Mitchell, Hugh D.
Orr, Galya
Markillie, Lye Meng
Jawdy, Sara S.
Grimwood, Jane
Shaw, A. Jonathan
Schmutz, Jeremy
Northen, Trent R.
Anderton, Christopher R.
Pelletier, Dale A.
Weston, David J.
Source :
The ISME Journal; 20240101, Issue: Preprints p1-12, 12p
Publication Year :
2024

Abstract

Interactions between Sphagnum(peat moss) and cyanobacteria play critical roles in terrestrial carbon and nitrogen cycling processes. Knowledge of the metabolites exchanged, the physiological processes involved, and the environmental conditions allowing the formation of symbiosis is important for a better understanding of the mechanisms underlying these interactions. In this study, we used a cross-feeding approach with spatially resolved metabolite profiling and metatranscriptomics to characterize the symbiosis between Sphagnumand Nostoccyanobacteria. A pH gradient study revealed that the Sphagnum–Nostocsymbiosis was driven by pH, with mutualism occurring only at low pH. Metabolic cross-feeding studies along with spatially resolved matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) identified trehalose as the main carbohydrate source released by Sphagnum, which were depleted by Nostocalong with sulfur-containing choline-O-sulfate, taurine and sulfoacetate. In exchange, Nostocincreased exudation of purines and amino acids. Metatranscriptome analysis indicated that Sphagnumhost defense was downregulated when in direct contact with the Nostocsymbiont, but not as a result of chemical contact alone. The observations in this study elucidated environmental, metabolic, and physiological underpinnings of the widespread plant–cyanobacterial symbioses with important implications for predicting carbon and nitrogen cycling in peatland ecosystems as well as the basis of general host-microbe interactions.

Details

Language :
English
ISSN :
17517362 and 17517370
Issue :
Preprints
Database :
Supplemental Index
Journal :
The ISME Journal
Publication Type :
Periodical
Accession number :
ejs58375839
Full Text :
https://doi.org/10.1038/s41396-021-01136-0