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Linear Epitopes Play an Important Role in the Immunoglobulin G (IgG)/Immunoglobulin E (IgE)-Binding Capacity of Scy p 4
- Source :
- Journal of Agricultural and Food Chemistry; November 2021, Vol. 69 Issue: 43 p12870-12879, 10p
- Publication Year :
- 2021
-
Abstract
- Sarcoplasmic calcium-binding protein is a stable allergen in Scylla paramamosainand named Scy p 4. To explore the importance of linear epitopes in the immunoglobulin G (IgG)/immunoglobulin E (IgE)-binding capacity of Scy p 4, chemical denaturants were used to destroy the structure. Scy p 4 was reduced with dithiothreitol and subsequently alkylated with iodoacetamide (IAA). Furthermore, the structural analysis indicated that IAA–Scy p 4 was an unstructured protein. The inhibition enzyme-linked immunosorbent assay showed that IAA–Scy p 4 could inhibit the binding of Scy p 4 to sensitize serum, with inhibition rates reached 55%. Moreover, the linear mimotopes of Scy p 4 were predicted in silico. Three linear epitopes were verified by serological tests and named L-Scy p 4-1 (AA76–91), L-Scy p 4-2 (AA111–125), and L-Scy p 4-3 (AA137–146). Overall, these data provide an understanding of the relationship between the structure and allergenicity about Scy p 4, and the identified linear epitopes can be used for diagnosis and food processing of shellfish allergy.
Details
- Language :
- English
- ISSN :
- 00218561 and 15205118
- Volume :
- 69
- Issue :
- 43
- Database :
- Supplemental Index
- Journal :
- Journal of Agricultural and Food Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs58117986
- Full Text :
- https://doi.org/10.1021/acs.jafc.1c05464