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Biophysical and Biochemical Heterogeneity of Purified Hepatitis B Antigen
- Source :
- The Journal of Virology; September 1972, Vol. 10 Issue: 3 p469-476, 8p
- Publication Year :
- 1972
-
Abstract
- Hepatitis B antigen of the D (a+, d+, y-) subtype was purified from plasma of apparently healthy persons and from hepatitis patients. The original samples contained 20- and 42-nm particles and tubular forms (20-nm diameter). Ultracentrifugation during the purification procedure yielded pellets which were then treated at pH 2.4. Both the large, 42-nm Dane particles and the tubular forms were lost during the acid treatment of the pelleted particles, yielding a preparation containing a mixture of particles approximately 20 and 25 nm in diameter. This difference in size was substantiated in that two distinct molecular weights were calculated from high-speed equilibrium data, 3.6 × 106and 4.5 × 106. Further heterogeneity was observed in that hepatitis B antigenic activity was present in purified particles with an isoelectric pH of 4.0 and also in those with a pH of 4.4. No significant differences were observed in the gross amino acid composition of purified antigen obtained from plasma of three different persons. 125I-labeled, purified antigen was found to contain six distinct polypeptides with molecular weights ranging from 10,000 to 39,000.
Details
- Language :
- English
- ISSN :
- 0022538X and 10985514
- Volume :
- 10
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- The Journal of Virology
- Publication Type :
- Periodical
- Accession number :
- ejs57815691
- Full Text :
- https://doi.org/10.1128/jvi.10.3.469-476.1972