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Molecular Characterization of the vanDGene Cluster and a Novel Insertion Element in a Vancomycin-Resistant Enterococcus Isolated in Canada

Authors :
Boyd, D. A.
Conly, J.
Dedier, H.
Peters, G.
Robertson, L.
Slater, E.
Mulvey, M. R.
Source :
Journal of Clinical Microbiology; June 2000, Vol. 38 Issue: 6 p2392-2394, 3p
Publication Year :
2000

Abstract

ABSTRACTA single vanD-containing Enterococcus faeciumstrain (N97-330) was isolated in Canada. ThevanD-containing region was cloned and sequenced. Although the proteins have more than 96% identity to a previously described vanDregion in BM4339, thevanSDgene contains a frameshift mutation that leads to a predicted truncated protein. Furthermore, sequence analysis of the ddlgene revealed the presence of an IS982-like element (ISEfm1) which interrupted the d-Ala–d-Ala ligase. This suggested the constitutive expression of the vanDoperon, which was confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated that BM4339 was not related to N97-330 (>15 band differences). Both strains contained multiple copies of the IS982-like element.

Details

Language :
English
ISSN :
00951137 and 1098660X
Volume :
38
Issue :
6
Database :
Supplemental Index
Journal :
Journal of Clinical Microbiology
Publication Type :
Periodical
Accession number :
ejs57778992
Full Text :
https://doi.org/10.1128/JCM.38.6.2392-2394.2000