Modified enzyme-linked immunosorbent assay for detecting enteroinvasive Escherichia coli and virulent Shigella strains

Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were absorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardless of species or serogroups. The investigation of 83 strains indicated complete agreement between enzyme-linked immunosorbent assay results and those of the keratoconjunctivitis test. It is assumed that the absorbed immune sera reacted with a possible virulence marker antigen. This inexpensive and simple method provides an alternative to other virulence tests. It has a definite advantage for screening large number of isolates within 24 h.