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Freeze-dried erythrocytes for an indirect hemagglutination test for detection of cytomegalovirus antibodies

Authors :
Cabau, N
Crainic, R
Duros, C
Denoyel, G
Gaspar, A
Bronnert, C
Boué, A
Horodniceanu, F
Source :
Journal of Clinical Microbiology; June 1981, Vol. 13 Issue: 6 p1026-1030, 5p
Publication Year :
1981

Abstract

An indirect hemagglutination test with lyophilized, fixed, tanned, and cytomegalovirus (CMV)-sensitized sheep erythrocytes for the detection of CMV antibodies is reported. To avoid nonspecific hemagglutination, cells were fixed with glutaraldehyde or Formalin directly in whole blood. The lyophilized, CMV-sensitized erythrocytes obtained by this technique were stable up to 9 months at 37 degrees C and retained the same reactivity at fresh, CMV-sensitized cells. Indirect hemagglutination performed with lyophilized, sensitized cells was highly efficient in detecting CMV-antibodies as compared with complement fixation and enzyme immunoassay.

Details

Language :
English
ISSN :
00951137 and 1098660X
Volume :
13
Issue :
6
Database :
Supplemental Index
Journal :
Journal of Clinical Microbiology
Publication Type :
Periodical
Accession number :
ejs57713424
Full Text :
https://doi.org/10.1128/jcm.13.6.1026-1030.1981