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Immunocytochemical localization of the coenzyme F420-reducing hydrogenase in Methanosarcina barkeri Fusaro
- Source :
- Journal of Bacteriology; February 1991, Vol. 173 Issue: 3 p978-984, 7p
- Publication Year :
- 1991
-
Abstract
- The cytological localization of the 8-hydroxy-5-deazaflavin (coenzyme F420)-reducing hydrogenase of Methanosarcina barkeri Fusaro was determined by immunoelectron microscopy, using a specific polyclonal rabbit antiserum raised against the homogeneous deazaflavin-dependent enzyme. In Western blot (immunoblot) experiments this antiserum reacted specifically with the native coenzyme F420-reducing hydrogenase, but did not cross-react with the coenzyme F420-nonreducing hydrogenase activity also detectable in crude extracts prepared from methanol-grown Methanosarcina cells. Immunogold labelling of ultrathin sections of anaerobically fixed methanol-grown cells from the exponential growth phase revealed that the coenzyme F420-reducing hydrogenase was predominantly located in the vicinity of the cytoplasmic membrane. From this result we concluded that the deazaflavin-dependent hydrogenase is associated with the cytoplasmic membrane in intact cells of M. barkeri during growth on methanol as the sole methanogenic substrate, and a possible role of this enzyme in the generation of the electrochemical proton gradient is discussed.
Details
- Language :
- English
- ISSN :
- 00219193 and 10985530
- Volume :
- 173
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Journal of Bacteriology
- Publication Type :
- Periodical
- Accession number :
- ejs57624870
- Full Text :
- https://doi.org/10.1128/jb.173.3.978-984.1991