The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthingene therapy

Plant-derived cytotoxic transgene expression, such as trichosanthin(tcs), regulated by recombinant adeno-associated virus (rAAV) vector is a promising cancer gene therapy. However, the cytotoxic transgene can hamper the vector production in the rAAV producer cell line, human embryonic kidney (HEK293) cells. Here, we explored microRNA-122 (miR122) and its target sequence to limit the expression of the cytotoxic gene in the rAAV producer cells.