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Temperature-sensitive catabolite activator protein in Escherichia coli BUG6

Authors :
Benner, D
Müller, N
Boos, W
Source :
Journal of Bacteriology; January 1985, Vol. 161 Issue: 1 p347-352, 6p
Publication Year :
1985

Abstract

BUG6 is a temperature-sensitive cell division mutant which forms filaments at the nonpermissive temperature. Synthesis of the maltose- and galactose-binding protein-dependent transport systems is also temperature sensitive in BUG6. Using operon and protein fusions of the maltose transport genes to lacZ, we observed that the temperature-sensitive control of the maltose transport system in BUG6 occurs at the transcriptional level. By P1-mediated transductions, we found that BUG6 contains two independent temperature-sensitive mutations. One maps between 2 and 3 min on the Escherichia coli linkage map, in close proximity to the fts-envA region. This mutation is responsible for temperature-sensitive cell division. The other mutation maps at 73 min in crp, the structural gene of the catabolite activator protein. The latter could be complemented by a hybrid plasmid carrying the wild-type crp as the only gene on a 0.9-kilobase HindIII-AluI restriction fragment. The mutation in crp alone was found to be responsible for the temperature-sensitive synthesis of the maltose transport system. Although it causes a complete block of transcription of the maltose transport genes at 41 degrees C, this mutation had only a marginal effect on the transcription of the lac operon.

Details

Language :
English
ISSN :
00219193 and 10985530
Volume :
161
Issue :
1
Database :
Supplemental Index
Journal :
Journal of Bacteriology
Publication Type :
Periodical
Accession number :
ejs57609697
Full Text :
https://doi.org/10.1128/jb.161.1.347-352.1985