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DNA supercoiling in gyrase mutants
- Source :
- Journal of Bacteriology; May 1984, Vol. 158 Issue: 2 p397-403, 7p
- Publication Year :
- 1984
-
Abstract
- Nucleoids isolated from Escherichia coli strains carrying temperature-sensitive gyrA or gyrB mutations were examined by sedimentation in ethidium bromide-containing sucrose density gradients. A shift to restrictive temperature resulted in nucleoid DNA relaxation in all of the mutant strains. Three of these mutants exhibited reversible nucleoid relaxation: when cultures incubated at restrictive temperature were cooled to 0 degree C over a 4- to 5-min period, supercoiling returned to levels observed with cells grown at permissive temperature. Incubation of these three mutants at restrictive temperature also caused nucleoid sedimentation rates to increase by about 50%.
Details
- Language :
- English
- ISSN :
- 00219193 and 10985530
- Volume :
- 158
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Journal of Bacteriology
- Publication Type :
- Periodical
- Accession number :
- ejs57609064
- Full Text :
- https://doi.org/10.1128/jb.158.2.397-403.1984