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Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12
- Source :
- Journal of Bacteriology; June 1978, Vol. 134 Issue: 3 p1117-1122, 6p
- Publication Year :
- 1978
-
Abstract
- Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI ends. Replication proceeded either unidirectionally or bidirectionally with equal frequency. Results of the analysis of replicative intermediates of RSF1010 containing the Apr-transposable sequence (Tn) are also presented.
Details
- Language :
- English
- ISSN :
- 00219193 and 10985530
- Volume :
- 134
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Journal of Bacteriology
- Publication Type :
- Periodical
- Accession number :
- ejs57599516
- Full Text :
- https://doi.org/10.1128/jb.134.3.1117-1122.1978