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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigeminaAntibodies in Cattle
- Source :
- Clinical and Vaccine Immunology (formerly CDLI); September 2008, Vol. 15 Issue: 9 p1316-1321, 6p
- Publication Year :
- 2008
-
Abstract
- ABSTRACTA competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigeminarhoptry-associated protein 1a was validated for international use. Receiver operating characteristic analysis revealed 16% inhibition as the threshold for a negative result, with an associated specificity of 98.3% and sensitivity of 94.7%. Increasing the threshold to 21% increased the specificity to 100% but modestly decreased the sensitivity to 87.2%. By using 21% inhibition, the positive predictive values ranged from 90.7% (10% prevalence) to 100% (95% prevalence) and the negative predictive values ranged from 97.0% (10% prevalence) to 48.2% (95% prevalence). The assay was able to detect serum antibody as early as 7 days after intravenous inoculation. The cELISA was distributed to five different laboratories along with a reference set of 100 defined bovine serum samples, including known positive, known negative, and field samples. The pairwise concordance among the five laboratories ranged from 100% to 97%, and all kappa values were above 0.8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application.
Details
- Language :
- English
- ISSN :
- 15566811 and 1556679X
- Volume :
- 15
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Clinical and Vaccine Immunology (formerly CDLI)
- Publication Type :
- Periodical
- Accession number :
- ejs57510221
- Full Text :
- https://doi.org/10.1128/CVI.00150-08