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Hemolysin plasmid coding for the virulence of a nephropathogenic Escherichia coli strain
- Source :
- Infection and Immunity; January 1982, Vol. 35 Issue: 1 p32-37, 6p
- Publication Year :
- 1982
-
Abstract
- The nephropathogenic Escherichia coli strain P673 was shown to harbor two plasmids with molecular sizes of 70 and 41 megadaltons, respectively. The 70-megadalton plasmid, pCW1, coded for tetracycline resistance, whereas hemolysin production was coded by the 41-megadalton plasmid, pCW2. Plasmid pCW1 proved to be self-transmissible, in contrast to pCW2. Transfer of the hemolysin character was associated with the appearance of a 110-megadalton plasmid, pCW3. The incompatibility of pCW3 with both native plasmids and restriction enzyme analysis led to the conclusion that pCW3 is a cointegrate of pCW1 and pCW2, pCW2, carrying the hemolytic determinant, is involved in the nephropathogenic character of strain P673, because (i) elimination of pCW2 from P673 was associated with a loss of virulence and (ii) the nephropathogenicity of the avirulent mutant could be restored by reintroduction of pCW2 DNA as part of a cointegrate structure.
Details
- Language :
- English
- ISSN :
- 00199567 and 10985522
- Volume :
- 35
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Infection and Immunity
- Publication Type :
- Periodical
- Accession number :
- ejs57488090
- Full Text :
- https://doi.org/10.1128/iai.35.1.32-37.1982