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Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4
- Source :
- Infection and Immunity; December 1981, Vol. 34 Issue: 3 p684-692, 9p
- Publication Year :
- 1981
-
Abstract
- Monoclonal antibodies were prepared against herpes simplex virus type 1 (strain 14012) by two immunization procedures. Procedure A utilized infectious virus propagated in mouse cells, and procedure B utilized mouse cells infected with herpes simplex virus in the presence of cycloheximide and harvested 1 h after removal of the inhibitor. A total of 52 monoclonal antibodies were obtained against 10 herpes simplex virus proteins, including four glycosylated proteins (a 110,000-molecular-weight protein, gB, gC, and gD) and six nonglycosylated proteins (a 68,000-molecular-weight protein, ICP 9, ICP 8, ICP 6, ICP 5, and the immediate-early ICP 4). The antibodies were assayed against herpes simplex virus types 1 and 2 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of radioimmunoprecipitates, immunofluorescence, and neutralization. Using the reagents prepared, we concluded that the 110,000-molecular-weight protein, gD, ICP 9, ICP 9, ICP 6, and the 68,000-molecular-weight protein express both type-specific and cross-reactive antigenic determinants. In contrast, nine antibodies against gB all cross-reacted with herpes simplex virus type 2, whereas eight antibodies to gC all reacted type specifically.
Details
- Language :
- English
- ISSN :
- 00199567 and 10985522
- Volume :
- 34
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Infection and Immunity
- Publication Type :
- Periodical
- Accession number :
- ejs57488028
- Full Text :
- https://doi.org/10.1128/iai.34.3.684-692.1981