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Self-Assembled Pea Protein Isolate Nanoparticles with Various Sizes: Explore the Formation Mechanism
- Source :
- Journal of Agricultural and Food Chemistry; September 2021, Vol. 69 Issue: 34 p9905-9914, 10p
- Publication Year :
- 2021
-
Abstract
- Pea protein isolate nanoparticles (PPINs) were successfully prepared by potassium metabisulfite (K2S2O5). The disulfide bonds were disrupted by K2S2O5, and then the PPINs were formed through self-assembly. The average diameter of PPINs increased from 124.7 to 297.5 nm as the concentration of K2S2O5was increased from 2 to 8 mM, and the PPINs showed higher ζ-potentials (−32.2 to −35.8 mV) and unimodal distribution. The content of free sulfhydryl groups first increased and then decreased with the fracture and reformation of disulfide bonds. Subsequently, the increase of the β-sheet, which has considerable hydrophobicity, promoted the formation of PPINs. The formation mechanism of PPINs was explored by dissociation tests: hydrophobic interactions maintained the basic skeleton of PPINs, disulfide bonds stabilized the internal structure, and hydrogen bonds existed on the exterior of the particles. This study provided a simple and economical method to fabricate nanoparticles.
Details
- Language :
- English
- ISSN :
- 00218561 and 15205118
- Volume :
- 69
- Issue :
- 34
- Database :
- Supplemental Index
- Journal :
- Journal of Agricultural and Food Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs57469882
- Full Text :
- https://doi.org/10.1021/acs.jafc.1c02105