Real-Time PCR Detection of gyrAand parCMutations in Streptococcus pneumoniae

ABSTRACTFluoroquinolone resistance in Streptococcus pneumoniaemainly involves stepwise mutations predominantly in the parCand gyrAgenes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.