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Analysis of T-cell responses to Aspergillus fumigatusantigens in healthy individuals and patients with hematologic malignancies
- Source :
- Blood; December 2002, Vol. 100 Issue: 13 p4521-4528, 8p
- Publication Year :
- 2002
-
Abstract
- Invasive aspergillosis has become a major cause of infection-related mortality in nonneutropenic patients after allogeneic stem cell transplantation (SCT). To assess the potential role ofAspergillus-specific T-cell responses for the successful control of invasive aspergillosis, lymphoproliferative responses toAspergillus fumigatusantigens were studied in healthy individuals, patients with evidence of invasive aspergillosis, and patients late after allogeneic SCT. In healthy individuals, a positive lymphoproliferative response was documented to cellular extracts of A fumigatus(14 of 16), the 88-kDa dipeptidylpeptidase (4 of 16), and the 90-kDa catalase (8 of 11). A predominant release of interferon γ (IFN-γ) in culture supernatants on stimulation with A fumigatusantigens was demonstrated in 13 of 17 healthy individuals, indicating a TH1 response. In patients with clinical evidence of invasive aspergillosis, a favorable response to antifungal therapy was found to correlate with a higher IFN-γ/interleukin 10 (IL-10) ratio in culture supernatants (n = 7; median ratio, IFN-γ/IL-10 = 1.0; range, 0.09-24.8) compared to 10 patients with progressive or stable disease (median ratio, IFN-γ/IL-10 = 0.1; range, 0.002-2.1; P= .04). Steroid treatment was found to suppressAspergillus-specific lymphoproliferation (P= .037) and release of IFN-γ in culture supernatants (P= .017). In contrast to cytomegalovirus- and tetanus toxoid–specific T-cell responses, Aspergillus-specific T-cell reconstitution late after allogeneic SCT was characterized by low stimulation indices and a low IFN-γ/IL-10 ratio. In addition, phosphoantigen-reactive Vγ9/Vδ2 T-cell clones from healthy individuals were found to produce significant amounts of tumor necrosis factor in response to A fumigatusantigens. In conclusion, these results further support the hypothesis that T cells contribute to the host defense against A fumigatus.
Details
- Language :
- English
- ISSN :
- 00064971 and 15280020
- Volume :
- 100
- Issue :
- 13
- Database :
- Supplemental Index
- Journal :
- Blood
- Publication Type :
- Periodical
- Accession number :
- ejs57131664
- Full Text :
- https://doi.org/10.1182/blood-2002-01-0265