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The recurrent SET-NUP214fusion as a new HOXAactivation mechanism in pediatric T-cell acute lymphoblastic leukemia

The recurrent SET-NUP214fusion as a new HOXAactivation mechanism in pediatric T-cell acute lymphoblastic leukemia

Authors :
Van Vlierberghe, Pieter
van Grotel, Martine
Tchinda, Joëlle
Lee, Charles
Beverloo, H. Berna
van der Spek, Peter J.
Stubbs, Andrew
Cools, Jan
Nagata, Kyosuke
Fornerod, Maarten
Buijs-Gladdines, Jessica
Horstmann, Martin
van Wering, Elisabeth R.
Soulier, Jean
Pieters, Rob
Meijerink, Jules P.P.
Source :
Blood; May 2008, Vol. 111 Issue: 9 p4668-4680, 13p
Publication Year :
2008

Abstract

T-cell acute lymphoblastic leukemia (T-ALL) is mostly characterized by specific chromosomal abnormalities, some occurring in a mutually exclusive manner that possibly delineate specific T-ALL subgroups. One subgroup, including MLL-rearranged, CALM-AF10or inv (7)(p15q34) patients, is characterized by elevated expression of HOXAgenes. Using a gene expression–based clustering analysis of 67 T-ALL cases with recurrent molecular genetic abnormalities and 25 samples lacking apparent aberrations, we identified 5 new patients with elevated HOXAlevels. Using microarray-based comparative genomic hybridization (array-CGH), a cryptic and recurrent deletion, del (9)(q34.11q34.13), was exclusively identified in 3 of these 5 patients. This deletion results in a conserved SET-NUP214 fusion product, which was also identified in the T-ALL cell line LOUCY. SET-NUP214 binds in the promoter regions of specific HOXAgenes, where it interacts with CRM1 and DOT1L, which may transcriptionally activate specific members of the HOXAcluster. Targeted inhibition of SET-NUP214 by siRNA abolished expression of HOXAgenes, inhibited proliferation, and induced differentiation in LOUCY but not in other T-ALL lines. We conclude that SET-NUP214 may contribute to the pathogenesis of T-ALL by enforcing T-cell differentiation arrest.

Details

Language :
English
ISSN :
00064971 and 15280020
Volume :
111
Issue :
9
Database :
Supplemental Index
Journal :
Blood
Publication Type :
Periodical
Accession number :
ejs57062459
Full Text :
https://doi.org/10.1182/blood-2007-09-111872