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Chronic lymphocytic leukemia preceded by cold agglutinin disease: intraclonal immunoglobulin light-chain diversity inVH4-34expressing single leukemic B cells
- Source :
- Blood; November 2002, Vol. 100 Issue: 9 p3419-3422, 4p
- Publication Year :
- 2002
-
Abstract
- Autoimmune phenomena may precede or accompany lymphoid malignancies, especially B-chronic lymphocytic leukemia (B-CLL). We report a patient with a 7-year history of primary (idiopathic) cold agglutinin (CA) disease in whom B-CLL subsequently developed. Immunophenotyping and single-cell reverse transcription–polymerase chain reaction (RT-PCR) were applied to investigate the origin and diversification of leukemic B cells. The obtained data indicate a memory cell-type origin of the B-CLL cells. Remarkably, theIgVκgenes of the B-CLL cells showed intraclonal diversity, whereas the mutational pattern of their paired IgVHgenes were invariant. Thus, the light-chain–restricted intraclonal diversity in individual leukemic B cells in this patient strongly indicates a differential regulation or selection of the ongoing mutational process. Of note, our findings suggest that this B-CLL had developed from the patient's CA-producing B-cell population.
Details
- Language :
- English
- ISSN :
- 00064971 and 15280020
- Volume :
- 100
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- Blood
- Publication Type :
- Periodical
- Accession number :
- ejs56991945
- Full Text :
- https://doi.org/10.1182/blood.V100.9.3419