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Laboratory Variants GESG170L, GESG170K, and GESG170HIncrease Carbapenem Hydrolysis and Confer Resistance to Clavulanic Acid
- Source :
- Antimicrobial Agents and Chemotherapy; 2021, Vol. 65 Issue: 6 pe01931-20-e01931-20, 200191190p
- Publication Year :
- 2021
-
Abstract
- The Guiana extended-spectrum (GES) β-lactamase GESG170H, GESG170L, and GESG170Kmutants showed kcat, Km, and kcat/Kmvalues very dissimilar to those of GES-1 and GES-5. The enhancement of the hydrolytic activity against carbapenems is potentially due to a shift of the substrate in the active site that provides better positioning of the deacylating water molecule caused by the presence of the imidazole ring of H170 and of the long side chain of K170 and L170.
Details
- Language :
- English
- ISSN :
- 00664804 and 10986596
- Volume :
- 65
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Antimicrobial Agents and Chemotherapy
- Publication Type :
- Periodical
- Accession number :
- ejs56372582
- Full Text :
- https://doi.org/10.1128/AAC.01931-20