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A Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity

Authors :
Tse, W. C.
Boger, D. L.
Source :
Accounts of Chemical Research; January 2004, Vol. 37 Issue: 1 p61-69, 9p
Publication Year :
2004

Abstract

A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.

Details

Language :
English
ISSN :
00014842 and 15204898
Volume :
37
Issue :
1
Database :
Supplemental Index
Journal :
Accounts of Chemical Research
Publication Type :
Periodical
Accession number :
ejs5603953