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Double Strand Breaks in DNA Inhibit Nucleotide Excision Repair in Vitro*
- Source :
- Journal of Biological Chemistry; November 1996, Vol. 271 Issue: 44 p27601-27607, 7p
- Publication Year :
- 1996
-
Abstract
- Nucleotide excision repair (NER) was measured in human cell extracts incubated with either supercoiled or linearized damaged plasmid DNA as repair substrate. NER, as quantified by the extent of repair synthesis activity, was reduced by up to 80% in the case of linearized plasmid DNA when compared with supercoiled DNA. An excess of undamaged linearized plasmid in the repair mixture did not interfere with DNA repair synthesis activity on a supercoiled damaged plasmid, indicating a cis-acting inhibiting effect.
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Volume :
- 271
- Issue :
- 44
- Database :
- Supplemental Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs55929637
- Full Text :
- https://doi.org/10.1074/jbc.271.44.27601