Back to Search Start Over

Distinct Roles for Leukemia Inhibitory Factor Receptor α-Chain and gp130 in Cell Type-specific Signal Transduction*

Authors :
Starr, Robyn
Novak, Ulrike
Willson, Tracy A.
Inglese, Melissa
Murphy, Vincent
Alexander, Warren S.
Metcalf, Donald
Nicola, Nicos A.
Hilton, Douglas J.
Ernst, Matthias
Source :
Journal of Biological Chemistry; August 1997, Vol. 272 Issue: 32 p19982-19986, 5p
Publication Year :
1997

Abstract

Leukemia inhibitory factor (LIF) induces a variety of disparate biological responses in different cell types. These responses are thought to be mediated through the functional LIF receptor (LIFR), consisting of a heterodimeric complex of LIFR α-chain (LIFRα) and gp130. The present study investigated the relative capacity of the cytoplasmic domains of each receptor subunit to signal particular responses in several cell types. To monitor the signaling potential of LIFRα and gp130 individually, we constructed chimeric receptors by linking the extracellular domain of granulocyte colony-stimulating factor receptor (GCSFR) to the transmembrane and cytoplasmic regions of either LIFRα or gp130. Both chimeric receptors and the full-length GCSFR in expressed in M1 myeloid leukemic cells to measure differentiation induction, in embryonic stem cells to measure differentiation inhibition, and in Ba/F3 cells to measure cell proliferation. Our results demonstrated that whereas GCSFR-gp130 receptor homodimer mediated a GCSF-induced signal in all three cell types, the GCSFR-LIFRα receptor homodimer was only functional in embryonic stem cells. These findings suggest that the signaling potential of gp130 and LIFRα cytoplasmic domains may differ depending upon the tissue and cellular response initiated.

Details

Language :
English
ISSN :
00219258 and 1083351X
Volume :
272
Issue :
32
Database :
Supplemental Index
Journal :
Journal of Biological Chemistry
Publication Type :
Periodical
Accession number :
ejs55832703
Full Text :
https://doi.org/10.1074/jbc.272.32.19982