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A reinvestigation of the amino acid sequences of bovine, rabbit, monkey, and human myelin basic proteins.
- Source :
- Journal of Biological Chemistry; January 1985, Vol. 260 Issue: 1 p472-474, 3p
- Publication Year :
- 1985
-
Abstract
- In order to determine whether bovine, rabbit, and monkey myelin basic proteins (BPs) have the sequence Gly-His or His-Gly at positions corresponding to bovine sequence 76-77, we isolated the tryptic peptides encompassing the sequence in question in these proteins and cleaved them into dipeptides with dipeptidyl aminopeptidase I (EC 3.4.14.1). Analysis by gas chromatography/mass spectrometry of the dipeptides released showed that in no case did His follow Gly or Gly precede His. The identification of peptides Ala-Gln and His-Gly (bovine BP) and Ser-His and Gly-Arg (rabbit and monkey BPs) established the His-Gly sequence. A similar sequence analysis of tryptic peptide (80-91) of human BP confirmed the sequence Thr-Gln-Asp-Glu-Asn-Pro (80-85).
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Volume :
- 260
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs55797032
- Full Text :
- https://doi.org/10.1016/S0021-9258(18)89756-3