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Expression of an Abelson murine leukemia virus-encoded protein in Escherichia coli causes extensive phosphorylation of tyrosine residues.
- Source :
- Journal of Biological Chemistry; November 1982, Vol. 257 Issue: 22 p13181-13184, 4p
- Publication Year :
- 1982
-
Abstract
- A segment of the Abelson murine leukemia virus (A-MuLV) genome was inserted into an Escherichia coli plasmid designed to allow the expression of the protein encoded by the viral gene. Bacteria expressing the A-MuLV-encoded protein were isolated; they had new phosphorylated proteins in which the phosphate was linked to tyrosine residues. These proteins included many that must be E. coli protein. One phosphotyrosine-containing protein of 62,000 molecular weight had reactivity with antiserum specific for authentic A-MuLV protein. The A-MuLV protein thus appears to be a tyrosine-specific protein kinase which is active in E. coli.
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Volume :
- 257
- Issue :
- 22
- Database :
- Supplemental Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs55611918
- Full Text :
- https://doi.org/10.1016/S0021-9258(18)33424-0