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Metabolic changes in mouse sperm during capacitation†.
- Source :
- Biology of Reproduction; October 2020, Vol. 103 Issue: 4 p791-801, 11p
- Publication Year :
- 2020
-
Abstract
- Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.
Details
- Language :
- English
- ISSN :
- 00063363 and 15297268
- Volume :
- 103
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Biology of Reproduction
- Publication Type :
- Periodical
- Accession number :
- ejs54443073
- Full Text :
- https://doi.org/10.1093/biolre/ioaa114