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5-hydroxymethyl-2-furaldehyde purified from Japanese pear (Pyrus pyrifoliaNakai cv. Nijisseiki) juice concentrate inhibits melanogenesis in B16 mouse melanoma cells
- Source :
- Bioscience, Biotechnology, and Biochemistry; November 2020, Vol. 84 Issue: 11 p2374-2384, 11p
- Publication Year :
- 2020
-
Abstract
- ABSTRACTPear juice concentrate prepared by boiling Japanese pear (Pyrus pyrifoliaNakai cv. Nijisseiki) juice can significantly inhibit the activity of tyrosinase, a key enzyme in melanin synthesis in human skin. Using the ethanol extract of pear juice concentrate, we homogeneously purified an active compound that was identified as 5-hydroxymethyl-2-furaldehyde (5-HMF) through 1H- and 13C-NMR and mass spectroscopy. We observed that 5-HMF inhibited the monophenolase and diphenolase activities of mushroom tyrosinase as a mixed-type inhibitor (Kivalues of 3.81 and 3.70 mmol/L, respectively). In B16 mouse melanoma cells, treatment with 170 µmol/L of 5-HMF significantly reduced α-melanocyte-stimulated melanin synthesis by suppressing the cyclic adenosine monophosphate-dependent signaling pathway involved in melanogenesis. The results of our study indicated that 5-HMF can be potentially used as a skin-lightening agent in the cosmetic industry.Abbreviations:AC: adenylate cyclase; CREB: cAMP response element-binding protein; dhFAME: S-(−)-10,11-Dihydroxyfarnesoic acid methyl ester; DMEM: dulbecco′s modified eagle medium; l-DOPA: 3-(3,4-Dihydroxyphenyl)- l-alanine; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HEPES: 4-(2-Hydroxyethyl)-1-piperazine ethane sulfonic acid; 5-HMF: 5-Hydroxymethyl-2-furaldehyde; MITF: microphthalmia-associated transcription factor; α-MSH: α-Melanocyte-stimulating hormone; PKA: protein kinase A; PVDF: polyvinylidene difluoride; SDS: sodium dodecyl sulfate; TRP1: tyrosinase-related protein 1; TRP2: tyrosinase-related protein 2
Details
- Language :
- English
- ISSN :
- 09168451 and 13476947
- Volume :
- 84
- Issue :
- 11
- Database :
- Supplemental Index
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Publication Type :
- Periodical
- Accession number :
- ejs54442089
- Full Text :
- https://doi.org/10.1080/09168451.2020.1792762